Flow citometry
Short description
BD FACSVerse, 3 lasers, 8 colours.
Applications
Life sciences
Expertise
Theoretical explanations and practical training about FACS Verse to bring the user to autonomy with possible remote or nearby assistance.
Assistance in the choice of fluorochromes, in the setting of compensations, in the gating strategy, in the technical interpretation of the results.
Reflection on
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morphological analysis (FSC, SSC)
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phenotyping (antibodies coupled to fluorochromes)
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DNA content (DAPI, PI...)
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Mitochondrial membrane potential (MitoTRACKER...)
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cell cycle (BrdU...)
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cell viability (PI, 7-AAD, Annexin V, SYTOX...)
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cells from cell lines or dissociated tissues and bacteria.
Technical description
3 lasers:
- Violet (405nm)
Filter 448/45 (BD V450, Pacific Blue, DAPI, SYTOX Blue...)
Filter 528/45 (BD V500, BD V510, AmCyan...)
- Blue (488nm)
Filter 527/32 (FITC, GFP, Alexa 488, SYTOX Green, BD B515...)
Filter 586/42 (PE, Propidium Iodide, DsRED...)
Filter 700/54 (PerCP, PerCP-Cy5, PE-Cy5, 7-AAD...)
Filter 783/56 (PE-Cy7...)
+ mcherry filter 613/18
- Red (635nm)
Filter 660/10 (APC, Alexa 647, Cy5...)
Filter 783/56 (APC-Cy7, APC-H7...)
Localization
Building xxx, Faculty of sciences, floor xxx, room #xxx
Contact for this microscope
Academic manager
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Technician
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 |
 |
 |
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Jean-Yves Matroule |
Kévin Willemart |
