MASS SPECTROMETRY

Expertise

Identification and characterization of small molecules, targetted compounds quantification (biomarkers, drugs...), proteomics analyses(gel-free or gel-dependent, label-free quantification), peptidomics (profile analyses), 2 dimensional HPLC (2D-LC), gel electrophoresis including 2D-DIGE.

Description

The University of Namur Mass Spectrometry platform (MaSUN) involves equipment from different research units of NARILIS, NAMEDIC and the University of Namur: the Department of Pharmacy, the Organic Chemistry Unit (UCO), the Mass Spectrometry service and the Cell Biology Research Unit of the Department of Biology URBC).

In 2014, NAMEDIC was awarded a FNRS grant to buy a new mass spectrometre, which now enables the quantification of small molecules in complex settings such as plasma.  This niew piece of equipment supplements the MaSUN actual service offer.

EQUIPMENT

 
The Proteomic platform is operated by an experienced technician, it is focused on gel-dependent proteomics and in particular on the 2D-DIGE (difference in gel electrophoresis) technology, relying on a combination of equipment including the IPGPhor, Ettan DALT, a 2D Optimizer for gradients, and the Typhoon Confocal scanner. 
This approach allows a comparative quantitative analysis of the proteome of a treated cell or tissue sample versus an untreated sample.

The MassSpec platform is operated by an experienced engineer, it combines different mass spectrometers (HCT ultra with ETD II, Bruker, coupled to a Dionex Ultimate 3000 nanoLC system, and a MaXis Bruker coupled to a Dionex Ultimate 3000 2D-LC system). Up to now the facility has been mainly dedicated to identify proteins separated by 2D-DIGE gels, based on the sequence of the peptides obtained after trypsinization.  Recently, gel-independent proteomics have started, relying only on the MassSpec facility.